Structural view of the 2A protease from human rhinovirus C15
نویسندگان
چکیده
منابع مشابه
Solution Structure of the 2A Protease from a Common Cold Agent, Human Rhinovirus C2, Strain W12
Human rhinovirus strains differ greatly in their virulence, and this has been correlated with the differing substrate specificity of the respective 2A protease (2Apro). Rhinoviruses use their 2Apro to cleave a spectrum of cellular proteins important to virus replication and anti-host activities. These enzymes share a chymotrypsin-like fold stabilized by a tetra-coordinated zinc ion. The catalyt...
متن کاملCloning and Expression of Protease 2A from Coxsakievirus B3
Protease 2A (2Apro) of coxsackievirus B3 (CVB3) plays a major role in viral replication. In case of infection, viral proteins are being synthesized from viral mRNA using host biosynthesis machinery. 2Apro of virus, after being synthesized, exhibits two critical functions, cleavage of viral proteins and breaking eukaryotic initiation factor 4G. The enzyme plays an essential role in viral replic...
متن کاملEnzymatic characterization of refolded human rhinovirus type 14 2A protease expressed in Escherichia coli.
Reported here is the production of recombinant human rhinovirus 14 (HRV14) 2A protease from bacterial cells transformed with a heat-inducible plasmid containing the HRV14 2A cDNA sequence. Overexpressed 2A protein partitioned into the inclusion bodies was solubilized in urea and then refolded in the presence of Zn2+ Transition metals were required for the restoration of 2A protease activity as ...
متن کاملcloning and expression of protease 2a from coxsakievirus b3
protease 2a (2apro) of coxsackievirus b3 (cvb3) plays a major role in viral replication. in case of infection, viral proteins are being synthesized from viral mrna using host biosynthesis machinery. 2apro of virus, after being synthesized, exhibits two critical functions, cleavage of viral proteins and breaking eukaryotic initiation factor 4g. the enzyme plays an essential role in viral replica...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology Communications
سال: 2018
ISSN: 2053-230X
DOI: 10.1107/s2053230x18003382